Transmission Electron Microscopy

Transmission Electron Microscopy

- In electron microscopes (EM) the images are formed by the interaction of electrons with the object.
- In transmission electron microscopes (TEM) electrons are transmitted through an ultra-thin specimen (usually 10 - 100nm thick).
- No surface representations, but images of the whole inner structure of the specimen.
- Usual magnifications: 1.000-1.000.000x

Resolution limits in life science area depend on the methodological approach.
Examples:
- Cryo-electron tomography: ~4 nm
- Negative staining of macromolecules: ~ 1.5 nm
- 3D cryo-electron microscopy of macromolecules: ~ 3 Å
- Electron crystallography of macromolecules: ~ 1 Å

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